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Caulobacter crescentus fatty acid-dependent cell cycle mutant.

D Hodgson, P Shaw, M O'Connell

    Journal of Bacteriology
    |April 1, 1984
    PubMed
    Summary

    A new Caulobacter crescentus mutant, AE6001, requires unsaturated fatty acids for growth. Without them, cell division and growth halt after DNA replication and flagellum assembly, indicating a role for the fatB503 gene in coordinating these processes.

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    Area of Science:

    • Microbiology
    • Cell Biology
    • Molecular Biology

    Background:

    • Caulobacter crescentus is a model organism for studying the cell cycle.
    • Fatty acid auxotrophs are essential for understanding lipid metabolism and its impact on cellular processes.
    • Membrane biogenesis is crucial for cell division and growth.

    Purpose of the Study:

    • To isolate and characterize a novel fatty acid auxotroph of Caulobacter crescentus.
    • To investigate the role of unsaturated fatty acids in cell cycle progression.
    • To elucidate the function of the fatB503 gene in coordinating DNA replication and membrane assembly.

    Main Methods:

    • Isolation and characterization of a fatty acid auxotroph (AE6001).
    • Cell cycle analysis under conditions of unsaturated fatty acid starvation and repletion.

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  • Construction and analysis of double mutants involving the fatB503 allele and membrane biogenesis mutations.

    • Main Results:

    • AE6001 requires unsaturated fatty acids for growth on glucose.
    • Unsaturated fatty acid starvation causes a cell cycle block at the predivisional stage, post-DNA replication and flagellum assembly.
    • Cell division and growth arrest are likely due to impaired membrane synthesis.
    • Addition of oleic acid rescues cell division and DNA replication initiation.
    • The fatB503 gene product appears to coordinate DNA replication and membrane assembly.

    Conclusions:

    • The fatB503 gene product plays a critical role in coordinating cell cycle events, specifically DNA replication and membrane biogenesis.
    • Unsaturated fatty acids are essential for Caulobacter crescentus cell cycle progression and membrane integrity.
    • This mutant provides a valuable tool for studying the intricate regulation of cell division.