Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic screen for cloned release factor genes.

R B Weiss, J P Murphy, J A Gallant

    Journal of Bacteriology
    |April 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Investigating the effect of synthetic nitrogen application rate on the milk production, intake, digestion, and nitrogen excretion of dairy cows fed perennial ryegrass or perennial ryegrass-white clover herbage.

    Journal of dairy science·2026
    Same author

    Editorial Expression of Concern: The NAD<sup>+</sup> salvage pathway modulates cancer cell viability via p73.

    Cell death and differentiation·2024
    Same author

    A preliminary study on the feasibility of two different cow-calf contact systems in a pasture-based, seasonal calving dairy system: effects on cow production and health.

    Animal : an international journal of animal bioscience·2024
    Same author

    Correction to: Mapping of a novel major effect Hessian fly field partial-resistance locus in southern soft red winter wheat line LA03136E71.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2023
    Same author

    Effect of feeding single-dam or pooled colostrum on maternally derived immunity in dairy calves.

    Journal of dairy science·2021
    Same author

    Restricting dairy cow access time to pasture in autumn: The effects on milk production, grazing behaviour and DM intake of late lactation dairy cows.

    Animal : an international journal of animal bioscience·2021
    Same journal

    Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

    Journal of bacteriology·2026
    Same journal

    Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

    Journal of bacteriology·2026
    Same journal

    DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

    Journal of bacteriology·2026
    Same journal

    Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

    Journal of bacteriology·2026
    Same journal

    Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

    Journal of bacteriology·2026
    Same journal

    YpeB stability affects germination possibly through delaying SleB activity in <i>Bacillus subtilis</i>.

    Journal of bacteriology·2026
    See all related articles

    Nonsense suppression screening uses competition between tRNA and release factors. This method confirmed release factor 2 and enabled cloning of release factor 1, advancing gene discovery.

    Area of Science:

    • Molecular Biology
    • Genetics

    Background:

    • Nonsense suppression is a biological process involving competition between transfer RNA (tRNA) and translational release factors.
    • This competition offers a potential mechanism for screening and identifying genes encoding these release factors.

    Purpose of the Study:

    • To investigate the competition between a nonsense suppressor tRNA and translational release factors.
    • To establish a screening method for identifying release factor genes.
    • To clone the gene for release factor 1.

    Main Methods:

    • Utilized nonsense suppression as a screening strategy.
    • Confirmed competition with the established gene for release factor 2.
    • Employed the screening method to isolate the gene for release factor 1.

    Related Experiment Videos

    Main Results:

    • Demonstrated that nonsense suppression accurately reflects competition between suppressor tRNA and release factors.
    • Successfully confirmed the expected competition involving release factor 2.
    • Successfully cloned the gene for release factor 1 using this screening approach.

    Conclusions:

    • Nonsense suppression provides a facile method for screening release factor genes.
    • The established competition mechanism is valid and applicable for gene cloning.
    • This study successfully cloned the release factor 1 gene, contributing to the understanding of translational machinery.