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Related Experiment Videos

Rapid immunofluorescent screening procedure using primary cell cultures or tissue sections.

A Furst, A P Mahowald

    Journal of Immunological Methods
    |May 11, 1984
    PubMed
    Summary

    This study introduces a fast immunofluorescent assay for screening hybridoma supernatants. The method uses sulfonated tissue culture cluster lids for efficient antibody detection and characterization against specific cell types.

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    Case of the month: What's your diagnosis?

    Schweizer Archiv fur Tierheilkunde·2018

    Area of Science:

    • Immunology
    • Cell Biology
    • Biotechnology

    Background:

    • Hybridoma technology is crucial for monoclonal antibody production.
    • Screening hybridoma supernatants for specific antibody binding is essential but can be time-consuming.
    • Current methods may require significant sample volumes and multiple assay steps.

    Purpose of the Study:

    • To develop a rapid and efficient immunofluorescent screening procedure for hybridoma supernatants.
    • To enable immediate detection and partial characterization of antibodies targeting specific cell types.
    • To streamline the antibody screening process using a single, versatile substrate.

    Main Methods:

    • Utilizing primary cell cultures grown on sulfonated tissue culture cluster lids as the substrate.

    Related Experiment Videos

  • Performing all antibody binding assay stages, including cell culture and microscopic observation, on the same substrate.
  • Employing condensation rings on lids to isolate individual cell groups for distinct assays.
  • Assaying both live and fixed cells with minimal culture supernatant volumes.

    • Main Results:

    • Demonstrated a rapid procedure for immunofluorescent screening of hybridoma supernatants.
    • Enabled immediate detection and partial characterization of antibodies binding selectively to specific cell types.
    • Showcased the utility of sulfonated cluster lids as a single substrate for the entire assay.

    Conclusions:

    • The described method offers a fast and efficient approach for hybridoma supernatant screening.
    • The use of sulfonated cluster lids simplifies the assay process and reduces reagent requirements.
    • This technique facilitates quicker identification and characterization of valuable monoclonal antibodies.