Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure of alpha 2-macroglobulin-protease complexes.

D Wang, A I Yuan, R D Feinman

    Annals of the New York Academy of Sciences
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pharmacokinetics and biodistribution of radioimmunoconjugates of anti-CD19 antibody and single-chain Fv for treatment of human B-cell malignancy.

    Cancer immunology, immunotherapy : CII·1998
    Same author

    Role of the alpha2-integrin in osteoblast-specific gene expression and activation of the Osf2 transcription factor.

    The Journal of biological chemistry·1998
    Same author

    Autocrine transforming growth factor alpha provides a growth advantage to malignant cells by facilitating re-entry into the cell cycle from suboptimal growth states.

    The Journal of biological chemistry·1998
    Same author

    Autocrine TGFalpha expression in the regulation of initiation of human colon carcinoma growth.

    Journal of cellular physiology·1998
    Same author

    Activation of nuclear factor-kappaB-dependent transcription by tumor necrosis factor-alpha is mediated through phosphorylation of RelA/p65 on serine 529.

    The Journal of biological chemistry·1998
    Same author

    Expression and cellular location of endothelin-1 mRNA in rat liver following endotoxemia.

    Chinese medical journal·1998

    Researchers analyzed thrombin-alpha 2M reaction mixtures using two-dimensional SDS-PAGE. Novel cross-linked species were identified, including divalent cross-linking of inhibitor chains and very high molecular weight complexes.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Proteomics

    Background:

    • Thrombin and alpha-2-macroglobulin (alpha 2M) form complexes.
    • Understanding the structure of these complexes is crucial for their biological function.
    • Previous studies have predicted or described certain complex structures.

    Purpose of the Study:

    • To analyze the molecular species formed in thrombin-alpha 2M reaction mixtures.
    • To identify and characterize novel complex structures.
    • To validate or refute proposed models of thrombin-inhibitor interactions.

    Main Methods:

    • Two-dimensional SDS-PAGE was employed to separate and analyze reaction mixtures.
    • Complex molecular species were identified based on their migration patterns.

    Related Experiment Videos

    Main Results:

    • Several probable molecular species within the thrombin-alpha 2M complexes were identified.
    • Two novel species were discovered: divalent cross-linking of inhibitor chains by a single enzyme molecule, and very high molecular weight species from intermolecular cross-linking.
    • Data did not support species with monovalent enzyme linkage to an intact subunit, though further study is needed.

    Conclusions:

    • Two-dimensional SDS-PAGE is effective for characterizing thrombin-alpha 2M complexes.
    • Novel cross-linked structures contribute to the complexity of thrombin-inhibitor interactions.
    • Further research is required to fully elucidate all possible thrombin-alpha 2M complex formations.