Polyamine regulation of stringent control in a polyamine-auxotrophic strain of Escherichia coli

Escherichia coli BGA8 , a mutant unable to synthesize putrescine, behaves as stringent or relaxed according to the presence or absence of polyamine, respectively, in the culture medium. The relaxed synthesis of RNA can be reverted back to stringent by addition of putrescine or spermidine. The stringent response depends on the concentration of the polyamine in the culture medium. The formation of guanosine 3'-diphosphate 5'-diphosphate elicited by amino acid starvation is stimulated at least 40-fold in putrescine-supplemented bacteria and only about 2-fold in putrescine-depleted cells.