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OK10-transformed cell lines: viral component generation and tumorigenicity
Intervirology
|January 1, 1984
Summary
Stable avian acute leukemia virus (ALV) nonproducer (NP) quail cell lines were created. Differences in tumorigenicity were observed between reverse-transcriptase-negative and -positive cell lines, despite both releasing noninfectious viral particles.
Area of Science:
- Virology
- Oncology
- Molecular Biology
Background:
- Avian acute leukemia virus OK10 can transform cells.
- Nonproducer (NP) cell lines release viral particles without being infectious.
- Understanding the transforming potential of viral proteins is crucial.
Purpose of the Study:
- To establish and characterize stable nonproducer (NP) quail cell lines transformed by avian acute leukemia virus OK10.
- To investigate the transforming protein (p200) of OK10.
- To compare the in vivo tumorigenicity of different NP cell lines.
Main Methods:
- Establishment of stable NP quail cell lines transformed by OK10.
- Analysis of viral particles released by NP cell lines (reverse transcriptase activity, gag proteins).
- Identification of the putative transforming protein p200 using in vitro translation and immunoprecipitation.
Main Results:
- Thirteen NP quail cell lines were established, all releasing noninfectious viral particles.
- The putative transforming protein p200 was identified in viral particles and cell extracts.
- Two NP clones, B5 (reverse-transcriptase-negative) and 9C (reverse-transcriptase-positive), showed distinct tumorigenicity in vivo, with 9C inducing tumors.
Conclusions:
- Stable NP cell lines can be generated from OK10-transformed quail cells.
- The transforming protein p200 is present in the released noninfectious particles.
- Differences in reverse transcriptase activity of viral particles correlate with in vivo tumorigenicity, suggesting complex mechanisms of transformation.