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Abortive infection of mumps virus in murine cell lines
Abstract:
Infection of L929 or BW5147 cells with mumps virus was shown to result in an abortive type of infection in that little or no progeny virus was produced. Both cell lines could adsorb mumps virus, indicating that restricted viral growth in these cells was not attributable to the absence of mumps virus receptors. Using [35S]methionine-labelled virus, it was demonstrated that restriction of virus growth in BW5147 cells was due in part to inefficient virus penetration into the cells. Virus-specific polypeptides were synthesized in mumps virus-infected L929 cells but were not detected in infected BW5147 cells. After addition of actinomycin D or anti-interferon serum to the cultures, mumps virus was able to replicate in L929 cells, whereas no virus growth was apparent in BW5147 cells.
Insights
Mumps virus infection causes abortive infections in L929 and BW5147 cells, with limited progeny. Restriction in BW5147 cells is partly due to poor virus entry and lack of viral protein synthesis.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Mumps virus infection typically leads to productive viral replication.
- Certain cell lines exhibit restricted viral growth upon mumps virus infection.
- Understanding host-pathogen interactions is crucial for controlling viral diseases.
Purpose of the Study:
- To investigate the mechanisms underlying abortive mumps virus infections in L929 and BW5147 cell lines.
- To determine the factors responsible for restricted viral replication in these specific cell types.
- To elucidate the role of viral entry and protein synthesis in abortive infections.
Main Methods:
- Infection of L929 and BW5147 cells with mumps virus.
- Assessment of viral adsorption using mumps virus receptors.
- Analysis of viral protein synthesis via [35S]methionine labeling.
- Evaluation of viral replication following treatment with actinomycin D or anti-interferon serum.
Main Results:
- Both L929 and BW5147 cells adsorbed mumps virus, indicating receptor presence.
- Restricted viral growth in BW5147 cells was partly attributed to inefficient virus penetration.
- Virus-specific polypeptides were synthesized in L929 cells but not in BW5147 cells.
- Mumps virus replicated in L929 cells after actinomycin D or anti-interferon serum addition, but not in BW5147 cells.
Conclusions:
- Mumps virus infection results in abortive outcomes in L929 and BW5147 cells.
- Inefficient viral entry and a block in viral protein synthesis contribute to restricted replication in BW5147 cells.
- Cellular factors, potentially related to interferon response, play a role in limiting mumps virus replication.