Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence of guinea pig myelin basic protein.

G E Deibler, R E Martenson, H C Krutzsch

    Journal of Neurochemistry
    |July 1, 1984
    PubMed
    Summary

    Researchers determined the guinea pig myelin basic protein sequence by comparing it to bovine protein. Key differences were found in the C-terminal region, including deletions and substitutions, resulting in a 167-amino acid protein.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Activator for soybean lipoxidase.

    Federation proceedings·2010
    Same author

    Effect of hemin proteins on soybean lipoxidase.

    Federation proceedings·2010
    Same author

    Complex nature of soybean lipoxidase.

    Federation proceedings·2010
    Same author

    Semenogelins are ectopically expressed in small cell lung carcinoma.

    Clinical cancer research : an official journal of the American Association for Cancer Research·2001
    Same author

    Structural requirements for hemoglobin to induce fibronectin receptor expression in Candida albicans.

    Biochemistry·2000
    Same author

    Cell contact-dependent activation of alpha3beta1 integrin modulates endothelial cell responses to thrombospondin-1.

    Molecular biology of the cell·2000

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Molecular Biology

    Background:

    • Myelin basic protein (MBP) is a critical component of the central nervous system's myelin sheath.
    • Understanding species-specific variations in MBP is essential for studying demyelinating diseases.

    Purpose of the Study:

    • To determine the tentative amino acid sequence of guinea pig myelin basic protein.
    • To identify sequence differences between guinea pig and bovine myelin basic protein.

    Main Methods:

    • Comparative analysis of tryptic peptide fragments from guinea pig and bovine myelin basic protein.
    • Amino acid sequence determination using peptide analysis.

    Main Results:

    • Confirmed known sequence differences in the N-terminal half of the molecule.
    • Identified specific alterations in the C-terminal half: missing Ser131, deleted Ala136-His137, Leu140 replaced by Phe, and an extra Ala insertion between Gly142 and His143.
    • Established the guinea pig MBP sequence comprises 167 amino acid residues with a molecular weight of 18,256.

    Conclusions:

    • The guinea pig myelin basic protein exhibits distinct sequence variations compared to its bovine counterpart, particularly in the C-terminal region.
    • These findings contribute to the detailed molecular characterization of myelin basic protein across species.
    • The identified sequence provides a foundation for further research into MBP function and its role in neurological conditions.

    Related Experiment Videos