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Monoclonal antibodies detect polymorphic determinants shared by I-A and I-E antigens
Molecular Immunology
|April 1, 1984
Summary
Monoclonal antibodies show cross-reactivity with mouse I-A and I-E antigens. One antibody recognizes the precursor E beta chain before its association with E alpha, indicating early determinant formation.
Area of Science:
- Immunogenetics
- Molecular immunology
- MHC research
Background:
- The Major Histocompatibility Complex (MHC) plays a crucial role in immune responses.
- Ia antigens, specifically I-A and I-E, are key components of the MHC involved in antigen presentation.
Purpose of the Study:
- To investigate the cross-reactivity of monoclonal antibodies (mAbs) with different Ia antigen products.
- To characterize the binding specificities of mAbs against I-A and I-E dimers and their precursors.
Main Methods:
- Utilized binding data from inbred mouse strains.
- Performed immunochemical isolation of Ia antigens.
- Separated antigens using non-reduced/reduced two-dimensional gel electrophoresis.
Main Results:
- Two monoclonal antibodies demonstrated cross-reactivity, binding to both A alpha A beta and E alpha E beta dimers.
- One mAb (K22-42) specifically reacted with the precursor E beta chain in B10.GD mice, which was associated with the invariant chain (Ii).
Conclusions:
- The study provides evidence for cross-reactivity among monoclonal antibodies targeting Ia antigens.
- The findings suggest that the determinant on the E beta chain is formed early, preceding its association with the E alpha chain.