Fine localization of sulphated and non-sulphated glycoconjugates in the rabbit oviduct during the estrous cycle

An ultrastructural histochemical investigation was carried out to ascertain the localization of the glucidie components and to verify the histochemical modifications during the physiological estrogenic cycle of rabbit oviduct. Stainings were performed with high iron diamine (HID), low iron diamine (LID), dialized iron (DI), tannic acid-uranyl acetate (TA-U), and periodate-thiocarbohydrazide-silver proteinate (PA-TCH-SP) methods. The findings indicated that the secretory activity is more intense in the isthmus than in the ampulla. Neutral glycoconjugates are localized both in the secreting elements of the ampulla and in those of the isthmus, while carboxylated and sulphated glycosaminoglycans are localized only in the secretory granules of the secreting elements of the isthmus.