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Related Experiment Videos

Cytogenetic effects of cyclamates.

E W Jemison, K Brown, B Rivers

    Advances in Experimental Medicine and Biology
    |January 1, 1984
    PubMed
    Summary

    Calcium cyclamate demonstrated significant in vitro effects on human lymphocytes, impairing DNA synthesis and increasing chromosome aberrations. These findings highlight potential impacts on leukocyte growth and morphology.

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    Area of Science:

    • In vitro toxicology
    • Cell biology
    • Genetics

    Background:

    • Calcium cyclamate is an artificial sweetener with debated health effects.
    • Human peripheral lymphocytes serve as a model for assessing chemical toxicity.
    • Previous studies on cyclamates have yielded inconclusive results regarding cellular effects.

    Purpose of the Study:

    • To investigate the in vitro cytogenetic and biochemical effects of calcium cyclamate on PHA-stimulated human peripheral lymphocytes.
    • To determine the impact of calcium cyclamate on DNA synthesis, chromosome morphology, and cell growth.
    • To assess the dose-dependent effects of calcium cyclamate on lymphocyte cultures.

    Main Methods:

    • Lymphocyte cultures were treated with varying concentrations of calcium cyclamate (10^-2 and 10^-3 M).
    • Techniques included autoradiography, cytological staining, cell counting, liquid scintillation, and karyotyping over 96 hours.
    • Morphological changes, mitotic rates, DNA synthesis (tritiated thymidine incorporation), and chromosome aberrations were analyzed.

    Main Results:

    • Calcium cyclamate exposure led to increased mitotic rates and enhanced stability of leukocyte cultures.
    • A significant decrease in deoxribonucleic acid (DNA) synthesis was observed, indicated by reduced tritiated thymidine incorporation.
    • Increased chromosome aberrations and notable morphological changes in lymphocytes were documented in the experimental group.

    Conclusions:

    • Calcium cyclamate exhibits variable but significant effects on human peripheral lymphocyte growth and chromosome morphology in vitro.
    • The compound impairs DNA synthesis and induces cytogenetic damage, suggesting potential toxicological concerns.
    • Further research is warranted to fully elucidate the long-term implications of calcium cyclamate exposure on cellular health.

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