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A new method for detecting histamine release.

P Stahl Skov, S Norn, B Weeke

    Agents and Actions
    |April 1, 1984
    PubMed
    Summary
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    A novel assay utilizes glass microfibers to measure basophil histamine release. This method offers high sensitivity and selectivity for histamine detection, simplifying allergy diagnostics.

    Area of Science:

    • Immunology
    • Allergy Diagnostics
    • Biochemistry

    Background:

    • Histamine is a key mediator in allergic reactions.
    • Accurate measurement of histamine release is crucial for diagnosing allergies.
    • Existing methods for histamine detection can be complex and time-consuming.

    Purpose of the Study:

    • To develop a new, sensitive, and selective assay for measuring histamine release.
    • To utilize glass microfibers as a solid phase for histamine capture.
    • To validate the performance of the new assay compared to conventional methods.

    Main Methods:

    • Glass microfibers were prepared and fixed to microtitre plates.
    • Washed blood was challenged with antigen to induce histamine release.
    • Released histamine was captured by glass microfibers.

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  • Fibre-bound histamine was quantified using the fluorometric o-phthaldialdehyde method.

    • Main Results:

    • The assay demonstrated high affinity and selectivity for histamine binding to glass microfibers.
    • Approximately 90% of released histamine was adsorbed to the microfibers.
    • The assay sensitivity was determined to be 0.63 ng histamine dihydrochloride.
    • Results closely matched conventional histamine release assays using isolated leukocytes.

    Conclusions:

    • Glass microfibers provide an effective solid phase for a sensitive histamine release assay.
    • This new method simplifies histamine measurement in allergy diagnostics.
    • The assay shows comparable results to established methods, indicating its reliability.