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Related Experiment Videos

PHI stimulates ACTH release from pituitary tumor cell.

S Heisler, R Veilleux, F Labrie

    Molecular and Cellular Endocrinology
    |May 1, 1984
    PubMed
    Summary

    Pituitary hormone-inhibiting peptide (PHI) weakly stimulates ACTH release from mouse pituitary cells, but not from rat pituitary cells, suggesting corticotrophs are not a primary target for PHI.

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    Area of Science:

    • Neuroendocrinology
    • Molecular Endocrinology
    • Cellular Signaling

    Background:

    • The peptide PHI (pituitary hormone-inhibiting peptide) was identified in the median eminence.
    • PHI's presence suggested a role in regulating anterior pituitary secretory function.
    • Anterior pituitary corticotrophs are responsible for releasing ACTH (adrenocorticotropic hormone).

    Purpose of the Study:

    • To investigate the effects of PHI on ACTH release from mouse pituitary corticotrophs.
    • To understand the signaling pathways involved in PHI's action on pituitary cells.
    • To determine if PHI is a significant modulator of ACTH secretion in the anterior pituitary.

    Main Methods:

    • Primary cultures of mouse pituitary corticotrophs were used to study PHI's effects.
    • Measurements of cyclic AMP accumulation and ACTH secretion in response to PHI.
    • Experiments using antagonists (nifedipine, dexamethasone) and modulators (somatostatin, oxotremorine) to block PHI's effects.

    Main Results:

    • PHI increased cyclic AMP accumulation and ACTH secretion in a concentration-dependent manner in mouse corticotrophs.
    • PHI was less potent than VIP (vasoactive intestinal peptide) in stimulating cyclic AMP and ACTH release.
    • PHI-induced ACTH secretion was inhibited by nifedipine, dexamethasone, somatostatin, and oxotremorine.
    • PHI did not elicit ACTH release from primary cultures of dispersed rat anterior pituitary cells.

    Conclusions:

    • PHI can stimulate ACTH secretion and cyclic AMP synthesis in mouse pituitary corticotrophs, but with lower potency than VIP.
    • The effects of PHI are mediated through pathways sensitive to calcium channel blockade and hormonal inhibition.
    • Corticotrophs are likely not a major target for PHI in the anterior pituitary, given its limited effect on rat pituitary cells and lower potency compared to VIP.

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