Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An abnormal plasma antithrombin with no apparent affinity for heparin.

J F Chasse, F Esnard, J D Guitton

    Thrombosis Research
    |May 15, 1984
    PubMed
    Summary

    A congenital antithrombin abnormality in a French family affects heparin binding, not overall concentration. This genetic condition impacts thrombin inactivation rates without causing thrombosis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Long-term outcome of liver transplantation in childhood: A study of 20-year survivors.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
    Same author

    Ovarian tumors in children and adolescents: a series of 41 cases.

    Diagnostic and interventional imaging·2014
    Same author

    Prenatal ultrasonographic detection of abdominal and pelvic tumors: impact on management.

    Pediatric surgery international·2013
    Same author

    Congenital portosystemic shunts in children: recognition, evaluation, and management.

    Seminars in liver disease·2013
    Same author

    The long-term outcome of hepatic artery thrombosis after liver transplantation in children: role of urgent revascularization.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2012
    Same author

    Pancreatic resections for solid or cystic pancreatic masses in children.

    Journal of pediatric gastroenterology and nutrition·2011

    Area of Science:

    • Biochemistry
    • Genetics
    • Hematology

    Background:

    • Congenital antithrombin deficiency can increase thrombotic risk.
    • Previous studies identified various molecular defects in antithrombin.

    Purpose of the Study:

    • To investigate a congenital antithrombin abnormality in a French family.
    • To characterize the functional and molecular properties of the affected antithrombin.

    Main Methods:

    • Assessed plasma antithrombin concentration and heparin cofactor activity.
    • Utilized gel-bound heparin to evaluate antithrombin binding.
    • Employed crossed immunoelectrophoresis with and without heparin.

    Main Results:

    • Affected individuals had normal plasma antithrombin concentration and thrombin inactivation rates without heparin.

    Related Experiment Videos

  • Heparin cofactor activity was reduced by approximately 50% in affected individuals.
  • Half of the plasma antithrombin failed to bind to heparin, and immunoelectrophoresis revealed altered heparin interaction.

    • Conclusions:

    • The identified molecular alteration specifically affects the heparin-binding site of antithrombin.
    • This defect impairs heparin's ability to enhance thrombin inactivation, despite normal antithrombin levels.