Skin porphyrin assay in porphyria

The analysis of skin porphyrins in biopsies in patients with porphyria cutanea tarda has been achieved by high pressure liquid chromatography. Porphyrins were determined as their methyl ester copper-chelates and 2-,4-,5-,6-,7- and 8-carboxylic porphyrins distinguished and quantified.