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Establishment of two chick embryo fibroblastic cell lines
Gan
|May 1, 1984
Summary
Chicken helper factor-negative chick embryo cells treated with MNNG developed neoplastic properties, forming colonies in soft agar but not tumors. Unaltered cells remained normal, indicating MNNG induced specific cellular changes.
Area of Science:
- Cell Biology
- Oncology
- Virology
Background:
- Chicken helper factor (chf)-negative chick embryo cells (CEC) are a model for studying cellular transformation.
- Chemical mutagens like N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) can induce neoplastic changes in cells.
Purpose of the Study:
- To investigate the effects of MNNG treatment on chf-negative CEC.
- To establish and characterize cell lines with altered growth properties.
Main Methods:
- Culturing chf-negative CEC with MNNG for over 400 passages.
- Establishing a control cell line (SPCC-OU1) without MNNG treatment.
- Assessing cellular morphology, growth in soft agar, and tumor formation in vivo.
Main Results:
- MNNG-treated cells (CHCC-OU1) exhibited piling up and colony formation in soft agar, indicative of transformation.
- SPCC-OU1 cells maintained normal morphology and did not form colonies in soft agar.
- Neither cell line produced tumors in syngeneic chickens or nude mice.
- Both cell lines produced avian endogenous leukosis virus of subgroup E.
Conclusions:
- MNNG treatment induced specific neoplastic characteristics in chf-negative CEC, including anchorage-independent growth.
- The transformed cells (CHCC-OU1) retained viral production but lost tumorigenicity in the tested models.
- These cell lines provide a model for studying chemical carcinogenesis and viral interactions in avian cells.