Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new method for fast blood cell counting and partial differentiation by flow cytometry.

G Valet

    Blut
    |August 1, 1984
    PubMed
    Summary

    A novel flow cytometry method enables rapid, simultaneous blood cell counting and biochemical analysis using specific stains. This approach offers earlier detection of pathologic cell conditions beyond traditional cell counts.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Potential of a cytomics top-down strategy for drug discovery.

    Current medicinal chemistry·2010
    Same author

    Cytomics, the human cytome project and systems biology: top-down resolution of the molecular biocomplexity of organisms by single cell analysis.

    Cell proliferation·2005
    Same author

    Immunophenotypic approach to the identification and characterization of clonal plasma cells from patients with monoclonal gammopathies.

    Journal of biological regulators and homeostatic agents·2005
    Same author

    Potential and challenges of a human cytome project.

    Journal of biological regulators and homeostatic agents·2004
    Same author

    Cytomics--new technologies: towards a human cytome project.

    Cytometry. Part A : the journal of the International Society for Analytical Cytology·2004
    Same author

    Past and present concepts in flow cytometry: a European perspective.

    Journal of biological regulators and homeostatic agents·2003

    Area of Science:

    • Hematology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Traditional blood cell counting methods lack biochemical analysis capabilities.
    • Existing methods require additional time for biochemical assessments.
    • Early detection of pathologic cell conditions is crucial for timely intervention.

    Purpose of the Study:

    • To introduce a new flow cytometry-based blood counting method.
    • To integrate absolute counts, relative proportions, and biochemical analysis in a single assay.
    • To reduce processing time for comprehensive blood analysis.

    Main Methods:

    • Utilized flow cytometry for simultaneous measurement of electrical cell volume, green fluorescence (DiOC6(3)), and RNA/DNA content (acridine orange).
    • Employed saline-diluted human or animal blood samples.
    • Staining time: 2-5 minutes; measuring time: 1-2 minutes.

    Main Results:

    • Successfully determined absolute counts and relative proportions of erythrocytes, reticulocytes, thrombocytes, lymphocytes, and granulocytes.
    • Simultaneously acquired electrical cell volume and fluorescence data.
    • Demonstrated the feasibility of combining blood cell counting with biochemical analysis.

    Conclusions:

    • The new method provides a rapid and comprehensive approach to blood analysis.
    • Integrating biochemical analysis with cell counting offers potential for earlier detection of disease.
    • Further automation of staining, measurement, and data evaluation is in progress.

    Related Experiment Videos