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Antigenic characterization of Pasteurella pneumotropica isolated from mice and rats
Abstract:
Antigenic characterization of P. pneumotropica derived from mice and rats were serologically investigated by use of hyperimmune rabbit sera against mouse strain M 1 and rat strain R 1 and absorbed sera m-1 and r-1 which were prepared by absorbing anti-M 1 serum with strain R 1 and anti-R 1 serum with strain M 1, respectively. All of 13 mouse strains employed were agglutinated with both anti-M 1 and anti-R 1 sera, but their agglutination titers with anti-M 1 serum were usually higher than those with anti-R 1 serum. Agglutination test of 13 rat strains with two antisera showed results converse to those of mouse strains. On the other hand, all the mouse strains were agglutinated with absorbed serum m-1 but not with absorbed serum r-1, while quite converse results were obtained with all the rat strains. Antigens reactive with the absorbed sera were remarkably destroyed by heating at 100 degrees C for 1 hour and treating with 1 N HCl, suggesting to be capsular antigens of bacterial cells.
Insights
This study serologically characterized Plesiomonas pneumotropica from mice and rats. Findings reveal distinct antigenic differences between mouse and rat strains, suggesting capsular antigens are involved.
Area of Science:
- Microbiology
- Immunology
- Bacteriology
Background:
- Plesiomonas pneumotropica is a bacterium found in rodents.
- Understanding its antigenic properties is crucial for diagnostics and research.
Purpose of the Study:
- To serologically characterize Plesiomonas pneumotropica strains from mice and rats.
- To identify specific antigens differentiating mouse and rat isolates.
Main Methods:
- Hyperimmune rabbit sera were prepared against specific mouse (M 1) and rat (R 1) strains.
- Absorbed sera (m-1 and r-1) were created by cross-absorption.
- Agglutination tests were performed on 13 mouse and 13 rat strains using these antisera.
Main Results:
- All mouse strains agglutinated with both anti-M 1 and anti-R 1 sera, with higher titers against anti-M 1.
- Rat strains showed converse agglutination patterns compared to mouse strains.
- Mouse strains reacted with absorbed serum m-1 but not r-1; rat strains showed the opposite reaction.
- Antigens were heat-labile and acid-labile, indicating a capsular nature.
Conclusions:
- Distinct antigenic profiles exist between mouse- and rat-derived Plesiomonas pneumotropica.
- Specific capsular antigens differentiate these strains.
- These findings aid in the serological classification of Plesiomonas pneumotropica.