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Related Experiment Videos

A cytolytic human T lymphocyte clone differentially recognizing HLA-B27 subtypes.

Y Miyachi, S L Wee, L K Chen

    Human Immunology
    |August 1, 1984
    PubMed
    Summary

    A new human T cell clone, F/M-F159, specifically targets HLA-B27 subtypes. This discovery advances understanding of HLA-B27

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    Area of Science:

    • Immunology
    • Genetics
    • Molecular Biology

    Background:

    • HLA-B27 is a human leukocyte antigen associated with various autoimmune diseases.
    • Understanding the specific epitopes recognized by T cells is crucial for dissecting disease pathogenesis.

    Purpose of the Study:

    • To produce and characterize a cytolytic human T cell (CTL) clone with specificity for HLA-B27 subtypes.
    • To investigate the fine specificity of T cell recognition for HLA-B27 molecules.

    Main Methods:

    • Generation of a cytolytic human T cell clone (F/M-F159).
    • Cell-mediated lympholysis (CML) assays using diverse target cells (unrelated donors, families, cell lines).
    • Blocking studies with anti-HLA-B27 monoclonal antibodies (e.g., B27M2).

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    Main Results:

    • The CTL clone F/M-F159 demonstrated specific lysis of HLA-B27 positive targets.
    • Differential lysis observed for B27+ targets reactive vs. non-reactive with B27M2 antibody.
    • Minimal lysis of HLA-B27 negative targets, including those expressing cross-reactive HLA-Bw47.

    Conclusions:

    • F/M-F159 recognizes a specific epitope on most serologically defined HLA-B27 molecules.
    • This epitope is distinct from, but related to, the one recognized by the B27M2 antibody.
    • Findings support a direct role for HLA-B27 in disease pathogenesis.