Three human cell surface antigen systems determined by genes on chromosome 12

Area of Science:

• Immunology
• Human Genetics
• Cell Biology

Background:

• Monoclonal antibodies are crucial tools for identifying and characterizing cell surface antigens.
• Understanding cell surface antigen distribution aids in cell type classification and disease research.

Purpose of the Study:

• To characterize novel mouse monoclonal antibodies (AbA123, AbA127, AbK152, AbM68, AbV1) raised against human tumor cells and melanocytes.
• To determine the molecular nature and chromosomal localization of the recognized cell surface antigens.

Main Methods:

• Immunization of mice with cultured human tumor cells and melanocytes to generate monoclonal antibodies.
• Serological typing of human cell lines and somatic cell hybrids using the generated antibodies.
• Analysis of antigen expression in relation to chromosome 12 deletions in somatic cell hybrids.

Main Results:

• Antibodies AbA123, AbA127, and AbK152 recognized a glycoprotein complex (A123/A127) on most human cells.
• Antigens M68 and V1, identified as glycolipids, showed distinct expression patterns.
• All three antigen systems (A123/A127, M68, V1) were unequivocally assigned to human chromosome 12.

Conclusions:

• The study successfully identified and characterized three new cell surface antigen systems (A123/A127, M68, V1).
• The expression of these antigens is determined by genes located on human chromosome 12.
• These findings represent novel genetic assignments to chromosome 12 and provide new markers for cell surface research.