Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Morpho-functional changes in normal cyclic endometrium.

G Pelusi, A Bondi, M P Morichelli

    Basic and Applied Histochemistry
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Emergence of Mycobacterium gordonae in heater-cooler units: a five-year prospective surveillance of devices frequently subjected to chloramine-T booster disinfection.

    The Journal of hospital infection·2024
    Same author

    The impact of COVID-19 pandemic control on vaccine-preventable invasive bacterial diseases in Piedmont (Italy).

    Infection·2022
    Same author

    Outbreak of ceftazidime-avibactam resistant Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae in a COVID-19 intensive care unit, Italy: urgent need for updated diagnostic protocols of surveillance cultures.

    The Journal of hospital infection·2022
    Same author

    Salivary gland tissues and derived primary and metastatic neoplasms: unusual pitfalls in the work-up of sellar lesions. A systematic review.

    Journal of endocrinological investigation·2021
    Same author

    Integrating rapid diagnostics in Gram-negative bloodstream infections of patients colonized by carbapenemase-producing Enterobacterales.

    The Journal of hospital infection·2021
    Same author

    Diffuse glioneuronal tumour with oligodendroglioma-like features and nuclear clusters (DGONC) - a molecularly defined glioneuronal CNS tumour class displaying recurrent monosomy 14.

    Neuropathology and applied neurobiology·2019
    Same journal

    Improved methods for the determination of 5-bromo-2-deoxyuridine labelling index in clones and colonies growing in plasma clots.

    Basic and applied histochemistry·1990
    Same journal

    Immunohistochemical localization of filaggrin in benign and malignant lesions of the human oral mucosa.

    Basic and applied histochemistry·1990
    Same journal

    LAK1: a novel leucocyte differentiation antigen shared by lymphoid and endothelial cells.

    Basic and applied histochemistry·1990
    Same journal

    Isobestic wavelength determination for succinic dehydrogenase-nitroblue tetrazolium-derived formazans in autonomic neurons.

    Basic and applied histochemistry·1990
    Same journal

    Absorbance scanning and systematic error.

    Basic and applied histochemistry·1990
    Same journal

    Cell kinetics of PHA-activated lymphocytes are slowed by prolonged hypertonic stress.

    Basic and applied histochemistry·1990
    See all related articles

    This study examined endometrial tissue during different cycle phases, revealing distinct patterns of acidic mucosubstances and epithelial membrane antigen (EMA) expression. These findings highlight dynamic changes in endometrial glycoproteins and EMA during the menstrual cycle.

    Area of Science:

    • Gynecologic Pathology
    • Histochemistry
    • Immunocytochemistry

    Background:

    • The endometrium undergoes cyclical changes throughout the menstrual cycle.
    • Understanding the biochemical and antigenic profile of the endometrium is crucial for reproductive health and disease diagnosis.

    Purpose of the Study:

    • To investigate the distribution of acidic mucosubstances and specific antigens (EMA, CEA, GCDFP-15) in normal human endometrium during the early proliferative and late secretory phases.
    • To elucidate potential cyclical variations in endometrial glandular secretions and cell surface markers.

    Main Methods:

    • Histochemical staining for acidic mucosubstances using Alcian blue.
    • Immunocytochemical analysis for epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA), and Gross Cyst Disease Fluid Protein-15 (GCDFP-15).

    Related Experiment Videos

  • Comparative analysis of endometrial tissue from the early proliferative and late secretory phases.

    • Main Results:

    • Alcian blue positive material (acidic mucosubstances) was present in both phases, with differential distribution.
    • Sulphated mucosubstances were exclusively detected in the secretory phase.
    • No immunoreactivity for CEA or GCDFP-15 was observed in either phase.
    • Epithelial membrane antigen (EMA) staining was positive in all cases, showing phase-dependent variations in distribution.

    Conclusions:

    • Endometrial mucosubstance production and EMA expression exhibit distinct patterns during the proliferative and secretory phases.
    • The presence of sulphated mucosubstances in the secretory phase suggests specific functional roles.
    • EMA distribution changes correlate with the cyclical transformation of the endometrium.