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Related Experiment Videos

A tissue-specific transcription enhancer element in the human immunoglobulin lambda light chain locus.

D A Spandidos, M L Anderson

    FEBS Letters
    |September 17, 1984
    PubMed
    Summary

    Researchers identified a tissue-specific transcriptional enhancer within the human immunoglobulin lambda light chain locus. This enhancer significantly boosts gene expression in myeloma cells but not in fibroblast cells.

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    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • The human immunoglobulin (Ig) lambda light chain locus contains constant region genes (Ke-Oz- and Ke-Oz+) crucial for antibody function.
    • Investigating regulatory elements like transcriptional enhancers is vital for understanding gene expression control.

    Purpose of the Study:

    • To determine the presence and function of a transcriptional enhancer within an 8.0-kb EcoRI fragment of the human Ig C lambda locus.
    • To assess the tissue-specific activity of this potential enhancer element.

    Main Methods:

    • Utilized recombinant plasmids linking the Ig fragment to the aminoglycoside phosphotransferase (aph) gene for transformation assays.
    • Employed RNA spot and Northern blot hybridization to quantify transient aph gene expression post-transfection.

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  • Tested activity in both mouse myeloma and mouse fibroblast cell lines.

    • Main Results:

    • In fibroblast cells, the Ig fragment did not influence transformation frequencies or aph gene expression levels.
    • In myeloma cells, the 8.0-kb Ig fragment demonstrated a tissue-specific enhancement, increasing transformants and aph gene expression by at least 10-fold.
    • These findings pinpoint a functional transcriptional enhancer within the human Ig C lambda locus.

    Conclusions:

    • An 8.0-kb EcoRI fragment of the human Ig C lambda locus contains a potent, tissue-specific transcriptional enhancer.
    • This enhancer preferentially activates gene expression in myeloma cells, suggesting a role in B-cell development or function.