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Membrane filter staining method: bacterial plate counts in 24 H.

K M Tse, C M Lewis

    Applied and Environmental Microbiology
    |August 1, 1984
    PubMed
    Summary
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    A new staining technique accurately counts bacterial colonies on membrane filters within 24 hours. This method is reliable for analyzing treated and untreated water samples quickly.

    Area of Science:

    • Microbiology
    • Water Quality Analysis
    • Bacteriology

    Background:

    • Accurate bacterial enumeration is crucial for assessing water safety.
    • Existing methods for bacterial plate counts can be time-consuming.
    • Rapid analysis is often required for water quality monitoring.

    Purpose of the Study:

    • To develop and validate a novel staining technique for bacterial colonies on membrane filters.
    • To establish a rapid and reliable method for bacterial plate counts.
    • To enable timely analysis of both treated and untreated water samples.

    Main Methods:

    • Development of a specific staining protocol for bacterial colonies grown on membrane filters.
    • Application of the staining technique to various water samples.

    Related Experiment Videos

  • Evaluation of the staining method for reliability and reproducibility of bacterial counts.

    • Main Results:

    • The described staining technique provides reliable and reproducible bacterial plate counts.
    • The procedure successfully quantifies bacterial colonies within a 24-hour timeframe.
    • The method is effective for both treated and untreated water samples.

    Conclusions:

    • The novel staining technique offers a rapid and dependable approach for bacterial enumeration in water.
    • This method facilitates prompt water quality analysis, enhancing public health surveillance.
    • The procedure is versatile and applicable to diverse water matrices requiring timely assessment.