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[Production of the hyporeactivity factor by mouse cells]
Biulleten' Eksperimental'Noi Biologii I Meditsiny
|September 1, 1984
Summary
Mouse cells L 929, when hyporeactive to repeated interferon induction, secrete a factor that suppresses further interferon production. This factor is distinct from interferon itself, which can also inhibit its own production.
Area of Science:
- Immunology
- Cell Biology
- Virology
Background:
- Interferon (IFN) is crucial for antiviral defense.
- Cellular responses to repeated IFN induction can lead to altered sensitivity (hyporeactivity).
- The mechanisms regulating IFN production during hyporeactivity are not fully understood.
Purpose of the Study:
- To investigate the factors involved in the hyporeactivity of L 929 mouse cells to repeated interferon induction.
- To determine if a secreted factor contributes to the suppression of interferon production during this state.
- To differentiate the role of this secreted factor from the potential autoinhibitory effects of interferon itself.
Main Methods:
- L 929 mouse fibroblast cell line was used.
- Cells were subjected to repeated induction of interferon using poly(I) x poly(C).
- Incubation medium was analyzed for factors influencing interferon production.
- Neutralization assays with antiserum against murine interferon were performed.
Main Results:
- L 929 cells in a hyporeactive state secreted a factor into the medium that suppressed interferon production.
- This secreted factor was not neutralized by antiserum against murine interferon, indicating it is not interferon itself.
- Interferon (IFN) was also found to potentially inhibit its own production, independent of the secreted factor.
Conclusions:
- A novel hyporeactivity factor secreted by L 929 cells actively suppresses interferon production.
- This secreted factor is distinct from murine interferon.
- Both the secreted factor and interferon itself can contribute to the regulation of interferon production during hyporeactivity.