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Inhibitory effect of mouse interferon on the growth of an embryonal carcinoma in mice
Abstract:
Embryonal carcinoma cells (F9) were resistant to the inhibitory effects of interferon (IFN) on viral multiplication and cell division in vitro. Nevertheless, daily administration of IFN resulted in a marked inhibition of the growth of this carcinoma injected intraperitoneally (ip) and increased mouse survival time. An incidental finding was that daily IFN treatment increased the serum level of alpha-fetoprotein in normal 129 mice.
Insights
Interferon (IFN) showed limited in vitro effects on F9 embryonal carcinoma cells but significantly inhibited tumor growth and improved survival in mice. IFN treatment also increased alpha-fetoprotein levels in normal mice.
Area of Science:
- Oncology
- Immunology
- Virology
Background:
- Embryonal carcinoma (EC) cells, such as F9, exhibit resistance to interferon (IFN) in vitro.
- This resistance extends to IFN's effects on viral multiplication and cellular division.
Purpose of the Study:
- To investigate the in vivo therapeutic potential of interferon (IFN) against F9 embryonal carcinoma.
- To assess the impact of daily IFN administration on tumor growth and survival in a murine model.
Main Methods:
- Daily intraperitoneal (ip) administration of IFN to mice bearing F9 embryonal carcinoma xenografts.
- Monitoring tumor growth inhibition and survival rates.
- Measurement of serum alpha-fetoprotein levels in normal mice.
Main Results:
- Daily IFN treatment markedly inhibited the growth of intraperitoneally injected F9 embryonal carcinoma.
- IFN administration led to increased survival time in tumor-bearing mice.
- An incidental finding was a significant increase in serum alpha-fetoprotein levels in normal 129 mice following daily IFN treatment.
Conclusions:
- Interferon (IFN) demonstrates significant in vivo anti-tumor efficacy against F9 embryonal carcinoma, despite in vitro resistance.
- IFN treatment improves survival outcomes in a murine model of embryonal carcinoma.
- IFN influences specific serum biomarkers like alpha-fetoprotein, suggesting broader physiological effects.