Induction of tolerance by haptenated liposomes carrying mouse erythrocyte membrane glycoprotein
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Membrane sialoglycoprotein (GP) of mouse erythrocytes were incorporated into artificial membranes of liposomes which also contained DNP-aminocaproylphosphatidylethanolamine hapten. The liposomes were tested for their potential to modulate the anti-hapten antibody response in mice as a plausible model of haptenated isologous erythrocytes which is known to be a potent tolerogen. Repeated intravenous inoculation with DNP-liposomes carrying mouse GP resulted in a significant suppression of splenic plaque-forming cells and serum antibody produced in the recipient mice against the subsequent immunization with DNP-KLH in saline or DNP-HGG in Freund's complete adjuvant, in a strictly hapten-specific fashion. The suppression was long-lasting, and the secondary response to DNP-KLH given over a month later was also affected. DNP-liposomes without GP, or those with rabbit GP, exhibited little or no suppressive effect. The observed capacity of mouse GP-bearing DNP-liposomes was reminiscent of that of haptenated mouse erythrocytes in that only the IgG response, but not the IgM response, was suppressed.
Membrane sialoglycoprotein (GP) of mouse erythrocytes were incorporated into artificial membranes of liposomes which also contained DNP-aminocaproylphosphatidylethanolamine hapten. The liposomes were tested for their potential to modulate the anti-hapten antibody response in mice as a plausible model of haptenated isologous erythrocytes which is known to be a potent tolerogen. Repeated intravenous inoculation with DNP-liposomes carrying mouse GP resulted in a significant suppression of splenic plaque-forming cells and serum antibody produced in the recipient mice against the subsequent immunization with DNP-KLH in saline or DNP-HGG in Freund's complete adjuvant, in a strictly hapten-specific fashion. The suppression was long-lasting, and the secondary response to DNP-KLH given over a month later was also affected. DNP-liposomes without GP, or those with rabbit GP, exhibited little or no suppressive effect. The observed capacity of mouse GP-bearing DNP-liposomes was reminiscent of that of haptenated mouse erythrocytes in that only the IgG response, but not the IgM response, was suppressed.