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Related Experiment Videos

Antibody responses to lambda 1J558 and lambda 2315 light chains. Specificity and genetic regulation.

B Bogen

    Scandinavian Journal of Immunology
    |November 1, 1984
    PubMed
    Summary

    BALB/c mice developed specific antibody responses to hidden determinants on free lambda chains of myeloma proteins. These immune responses varied based on the specific lambda chain and immunization method, highlighting technical considerations for antibody detection.

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    Autologous bone marrow Th cells can support multiple myeloma cell proliferation in vitro and in xenografted mice.

    Leukemia·2017

    Area of Science:

    • Immunology
    • Protein Chemistry
    • Biochemistry

    Background:

    • Myeloma proteins are immunoglobulin proteins produced by a single clone of plasma cells.
    • Antibody responses are crucial for adaptive immunity and can be directed against various protein determinants.
    • Understanding antibody specificity is vital for diagnostics and therapeutics.

    Purpose of the Study:

    • To investigate the specificity of BALB/c antibody responses to lambda light chains of isologous myeloma proteins 315 and J558.
    • To explore the impact of chain assembly and immunization strategy on antibody recognition of lambda chain determinants.
    • To identify potential technical artifacts in antibody binding assays.

    Main Methods:

    • Enzyme-linked immunosorbent assay (ELISA) was employed to detect and quantify antibody binding.

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  • Immunization strategies involved using assembled (heavy + light chains) and free lambda myeloma proteins.
  • Antibody cross-reactivity and binding to specific regions (variable and constant) were analyzed.

    • Main Results:

    • Free lambda 2(315) immunization elicited high-titred IgG antibodies recognizing 'hidden' determinants, with some cross-reactivity to other lambda chains.
    • Free lambda 1J558 immunization induced low-titred IgM antibodies against 'hidden' constant region determinants, with no cross-reactivity.
    • Myeloma protein coating directly to polystyrene surfaces artefactualy exposed lambda chain determinants, an effect mitigated by spacer molecules.

    Conclusions:

    • Antibody responses to free lambda chains target 'hidden' determinants, with specificity influenced by the protein and immunization route.
    • The study identified a technical artifact in lambda chain determinant exposure during ELISA, emphasizing the importance of assay design.
    • Covariation of antibody and T helper cell responses differed between lambda 2(315) and lambda 1J558, suggesting distinct immune regulation pathways.