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Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells
Abstract:
Platelet-derived growth factor (PDGF) has been previously shown to be homologous to the transforming gene of simian sarcoma virus (v-sis), and inappropriate expression of the cellular counterpart of the v-sis gene (c-sis) has been implicated in the generation of mesenchymal tumors. The U-2 OS human osteosarcoma line was shown to contain multiple c-sis transcripts. Immunoprecipitation experiments with antiserum to PDGF identified a variety of polypeptides ranging in size from 18,000 to 165,000 daltons that were immunoprecipitated specifically from U-2 OS cell extracts. The osteosarcoma also was shown to secrete a 29,000-dalton protein having the serological and structural characteristics of PDGF.
Insights
This study found that U-2 OS osteosarcoma cells express multiple platelet-derived growth factor (PDGF) transcripts and secrete a PDGF-like protein. This suggests a role for PDGF in osteosarcoma development.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Platelet-derived growth factor (PDGF) shares homology with the simian sarcoma virus transforming gene (v-sis).
- Aberrant expression of the cellular v-sis gene (c-sis) is linked to mesenchymal tumor formation.
Purpose of the Study:
- To investigate the expression of c-sis transcripts in the U-2 OS human osteosarcoma cell line.
- To determine if U-2 OS cells produce and secrete PDGF-like proteins.
Main Methods:
- Analysis of c-sis transcript levels in U-2 OS cells.
- Immunoprecipitation using anti-PDGF antiserum to identify and characterize secreted proteins.
Main Results:
- U-2 OS cells contain multiple c-sis transcripts.
- Immunoprecipitation detected various polypeptides (18-165 kDa) in U-2 OS cell extracts.
- A 29 kDa protein with PDGF characteristics was secreted by the osteosarcoma cells.
Conclusions:
- The U-2 OS osteosarcoma cell line expresses c-sis and secretes a protein with PDGF properties.
- These findings suggest a potential role for PDGF in the pathogenesis of osteosarcoma.