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[Lymphocyte separation with dextran M 70].

H W Mansfeld, D Götze, A Ittenson

Allergie Und Immunologie

|January 1, 1984

Summary

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Immunogenetics·2011

Dextran M 70 can replace Ficoll 400 for isolating lymphocytes from human blood. This study found no significant differences in cell yield, vitality, or lymphocyte subsets when using Dextran M 70 in density gradient centrifugation.

Area of Science:

Immunology
Cell Biology
Biochemistry

Background:

Ficoll 400 is commonly used for density gradient centrifugation to separate lymphocytes.
Alternative reagents are sought to optimize cell separation protocols.

Purpose of the Study:

To evaluate Dextran M 70 as a substitute for Ficoll 400 in lymphocyte separation.
To compare the efficacy of Dextran M 70 and Ficoll 400 in density gradient centrifugation.

Main Methods:

Density gradient centrifugation of human peripheral blood.
Separation of mononuclear cells using Dextran M 70 and Visotrast 370.
Comparison with Ficoll 400 and Visotrast 370.
Analysis of lymphocyte yield, vitality, B-lymphocyte (surface immunoglobulins), and T-lymphocyte (rosette assay, BL-T2) counts.

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Main Results:

No significant differences were observed in lymphocyte yield or vitality between Dextran M 70 and Ficoll 400.
The numbers of B-lymphocytes and T-lymphocytes remained comparable when using either Dextran M 70 or Ficoll 400.
The study assessed specific markers including surface immunoglobulins, sheep red blood cells-rosette assay, and the monoclonal antibody BL-T2.

Conclusions:

Dextran M 70 is a viable alternative to Ficoll 400 for separating mononuclear cells from human peripheral blood.
The findings suggest Dextran M 70 can be used without compromising key lymphocyte parameters.
This provides a potential cost-effective or alternative option for lymphocyte isolation techniques.