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Aspergillus contains multiple tubulin genes.

J A Weatherbee, N R Morris

    The Journal of Biological Chemistry
    |December 25, 1984
    PubMed
    Summary

    Aspergillus nidulans contains at least two genes for beta-tubulin, identified through benomyl-resistant mutants. Further analysis revealed an additional beta-tubulin (beta 3), suggesting multiple beta-tubulin genes in Aspergillus.

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    The LIS1-related protein NUDF of Aspergillus nidulans and its interaction partner NUDE bind directly to specific subunits of dynein and dynactin and to alpha- and gamma-tubulin.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Cell Biology

    Background:

    • The benA locus in Aspergillus nidulans is a known structural gene for beta-tubulin.
    • Benomyl-resistant mutants have previously been used to study tubulin function.

    Purpose of the Study:

    • To investigate the presence of additional beta-tubulin genes in Aspergillus nidulans.
    • To characterize a newly identified beta-tubulin spot in electrophoretic gels.

    Main Methods:

    • Analysis of benomyl-resistant Aspergillus nidulans mutants (benA22, benA85).
    • Two-dimensional gel electrophoresis to separate and visualize tubulin proteins.
    • Partial purification of tubulin for further analysis.
    • Immunoblotting using anti-tubulin antibodies.
    • One-dimensional peptide mapping.

    Main Results:

    • Mutants benA22 and benA85 exhibited altered electrophoretic mobilities for beta-tubulins beta 1 and beta 2.
    • A previously occluded spot, identified as beta-tubulin 3, was revealed by these shifts.
    • Beta-tubulin 3 was confirmed through immunoblotting and peptide mapping.
    • Evidence suggests at least two structural genes for alpha-tubulins also exist in Aspergillus.

    Conclusions:

    • Aspergillus nidulans possesses at least two distinct structural genes encoding beta-tubulins.
    • The study identified and characterized a third beta-tubulin, designated beta 3.
    • These findings contribute to understanding the genetic basis of tubulin diversity in eukaryotes.

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