Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specialized transduction with lambda plac5: dependence on recB.

R D Porter, R A Welliver, T A Witkowski

    Journal of Bacteriology
    |June 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Core competencies in ethics.

    The Journal of health administration education·2002
    Same author

    Rapacuronium: first experience in clinical practice.

    European journal of anaesthesiology. Supplement·2002
    Same author

    Armed services vocational aptitude battery predictors of entry-level radiography students' success.

    Military medicine·2001
    Same author

    Identification of amino acids stabilizing the tetramerization of the single stranded DNA binding protein from Escherichia coli.

    FEBS letters·1998
    Same author

    Air Force School of Health Care Science's quality of life study.

    Military medicine·1998
    Same author

    Predictors of students' success in the Air Force School of Health Care Sciences.

    Military medicine·1998
    Same journal

    The bacterial SOS response promotes the expression of the transposase encoded by IS<i>CR</i> mobile genetic elements.

    Journal of bacteriology·2026
    Same journal

    Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

    Journal of bacteriology·2026
    Same journal

    Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

    Journal of bacteriology·2026
    Same journal

    DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

    Journal of bacteriology·2026
    Same journal

    Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

    Journal of bacteriology·2026
    Same journal

    Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

    Journal of bacteriology·2026
    See all related articles

    This study investigated recB dependence in specialized transduction. Results show recB is crucial for F42 lac recombination but not chromosomal lac recombination, impacting transduction frequency and outcomes.

    Area of Science:

    • Molecular Biology
    • Microbiology
    • Genetics

    Background:

    • Specialized transduction is a key mechanism for gene transfer mediated by bacteriophages.
    • The recB gene product plays a critical role in DNA repair and recombination pathways in bacteria.

    Purpose of the Study:

    • To investigate the role of the recB gene in the process of specialized transduction.
    • To determine if recB dependence varies based on the nature of the recipient DNA substrate.

    Main Methods:

    • Utilized genetically disabled lambda plac5 transducing phage derivatives.
    • Normalized transduction frequency to colony-forming units.
    • Scored for addition and substitution transductants to monitor recombination end products.

    Main Results:

    Related Experiment Videos

    • RecB dependence was minimal when using a chromosomal lac gene as the recipient DNA.
    • A significant recB dependence was observed for both transduction frequency and recombination products when using F42 lac as the recipient.
    • RecB showed no significant role in recombination within addition transductants involving two lac regions.
    • UV irradiation of phages increased transductant levels but altered the addition/substitution ratio for chromosomal lac.

    Conclusions:

    • The recB gene's role in specialized transduction is substrate-dependent.
    • RecB is essential for efficient recombination involving F42 lac, but less so for chromosomal lac.
    • These findings elucidate the differential involvement of recB in bacteriophage-mediated recombination pathways.