Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alkaline phosphatase activity in human polymorphonuclear leukocytes.

M Borgers, F Thoné, J de Crée

    The Histochemical Journal
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Response to exercise and mechanical efficiency in non-ischaemic stunning, induced by short-term rapid pacing in dogs: a role for calcium?

    Acta physiologica (Oxford, England)·2015
    Same author

    Ultrastructural calcium distribution and myocardial calcium content in human idiopathic dilated cardiomyopathy.

    Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
    Same author

    Chronic ischemic viable myocardium in man: Aspects of dedifferentiation.

    Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
    Same author

    Contact sites between the inner and outer mitochondrial membranes in stunned versus hibernating myocardium.

    Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
    Same author

    Chronic hibernating myocardium in sheep can occur without degenerating events and is reversed after revascularization.

    Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2014
    Same author

    Use of the ECG-triggered Venous Occlusion Plethysmograph to Study Hyperæmic Response Patterns in Patients with Intermittent Claudication treated with Cinnarizine.

    Proceedings of the Royal Society of Medicine·2010

    Alkaline phosphatase ultracytochemistry in human neutrophils (PMN) reveals plasma membrane and vacuole staining, not specific granules. Isolation methods and cancer influence enzyme activity distribution.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Immunohistochemistry

    Background:

    • Alkaline phosphatase (AP) is an enzyme with diverse cellular functions.
    • Its precise localization within human neutrophils (PMN) has been debated.
    • Previous studies show conflicting data regarding AP distribution in PMN.

    Purpose of the Study:

    • To ultracytochemically localize alkaline phosphatase in human neutrophils.
    • To investigate the influence of isolation procedures on AP localization.
    • To examine AP distribution in PMN from cancer patients.

    Main Methods:

    • Ultracytochemistry was used to localize alkaline phosphatase activity.
    • Human neutrophils (PMN) with normal and elevated AP levels were studied.
    • Isolation procedures involving dextran and Ficoll-Hypaque were evaluated.

    Related Experiment Videos

    Main Results:

    • Alkaline phosphatase activity was localized to the plasma membrane and endocytic vacuole membranes, not specific granules.
    • Dextran and Ficoll-Hypaque isolation methods enhanced AP staining on the PMN plasma membrane.
    • PMN from cancer patients showed increased AP activity on the plasma membrane, atypical vesicles, and sometimes the Golgi apparatus.

    Conclusions:

    • The distribution of alkaline phosphatase in human PMN is dependent on isolation techniques.
    • Elevated AP activity and altered localization are observed in PMN from cancer patients.
    • These findings help reconcile controversial data on AP distribution in human PMN.