Factors affecting the analysis of cefoperazone in cerebral spinal fluid

The measurement of cefoperazone concentrations in cerebral spinal fluid (CSF) is important to understanding the biodisposition and pharmacokinetic behavior of the drug. We have demonstrated that cefoperazone is unstable in methanol and at alkaline pH and these factors may affect the accurate quantitation of the drug in CSF. Buffering CSF samples with acetate is recommended to improve cefoperazone stability in CSF.