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Related Experiment Videos

Allogeneic responses in vitro induced by fetomaternal alloimmunization.

N Genetet, B Genetet, V Amice

    American Journal of Reproductive Immunology : AJRI : Official Journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction
    |April 1, 1982
    PubMed
    Summary

    Multiparous women with antibodies against paternal HLA-DR antigens often show unresponsiveness in mixed lymphocyte culture (MLC) tests. This suggests pregnancy induces suppressor cells or blocking antibodies that modulate immune responses during gestation.

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    Area of Science:

    • Immunology
    • Reproductive Immunology

    Background:

    • Pregnancy involves complex immune interactions between mother and fetus.
    • Multiparous women (MW) with antibodies against paternal HLA-DR antigens present a unique model to study these interactions.
    • Mixed lymphocyte culture (MLC) is a standard method for assessing immune reactivity.

    Purpose of the Study:

    • To investigate how pregnancy-induced allogeneic reactions affect MLC reactivity in multiparous women.
    • To identify the mechanisms behind altered immune responses during pregnancy, specifically in relation to HLA-DR antigens.

    Main Methods:

    • Utilized mixed lymphocyte culture (MLC) for HLA-D typing of lymphocytes from multiparous women.
    • Conducted kinetic experiments to analyze the timing of immune responses.
    • Performed direct cytotoxicity assays.

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  • Analyzed supernates for blocking activity and assessed suppressor cell activity in vitro.

    • Main Results:

    • Frequent unresponsiveness to homozygous typing cells (HTC) representing paternal/fetal HLA-DR determinants was observed.
    • Kinetics experiments excluded early secondary proliferative responses as the cause of unresponsiveness.
    • Direct cytotoxicity did not consistently correlate with proliferative response inhibition.
    • Evidence suggests specific anti-HLA-DR blocking antibodies and/or suppressor cells contribute to the observed immune modulation.

    Conclusions:

    • Pregnancy can induce selective suppressor cells and/or blocking antibodies in multiparous women.
    • These mechanisms appear to modulate immune responses, potentially protecting the fetus from maternal rejection.
    • Suggests the induction of auto-regulator mechanisms during pregnancy to manage antibody production and immune tolerance.