Secondary acrylamides in Nyloprint printing plate as a source of contact dermatitis

Several chemically pure fractions have been isolated from Nyloprint printing plates. 5 of the fractions elicited positive patch test reactions in 7 Nylonprint allergic printers. 4 of the 5 components were analysed by means of nuclear magnetic resonance, infrared spectrophotometry, mass spectrometry and elemental analysis. The fractions were identified as N,N'-methylene-bis-acrylamide. N,N'-[oxybis(methylene)]bis-2-propenamide, N,N'-[1,2-ethanediylbis(oxymethylene)]-2-propenamide, and a new compound N-[(2-hydroxyethoxy)-methyl]-2-propenamide. All of the analysed fractions have the common feature of being secondary acrylamides.