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Immunoradiometric assay of salivary anti-c3 antibodies
By an immunoradiometric assay IgA and IgG anti-C3 antibodies were found in adult parotid salivas. The binding of the IgA antibodies to complement coated red cells could be reversed by washing in buffer containing 0.01 M EDTA but IgG antibodies were unaffected. The binding of IgA antibodies was inhibited by small quantities of purified C3. This assay may prove useful in the detection of anti-C3 antibodies in other body fluids.
By an immunoradiometric assay IgA and IgG anti-C3 antibodies were found in adult parotid salivas. The binding of the IgA antibodies to complement coated red cells could be reversed by washing in buffer containing 0.01 M EDTA but IgG antibodies were unaffected. The binding of IgA antibodies was inhibited by small quantities of purified C3. This assay may prove useful in the detection of anti-C3 antibodies in other body fluids.