Related Experiment Videos
[Dye exclusion test and LDH release assay (author's transl)]
Allergie Und Immunologie
|January 1, 1982
Summary
The lactate dehydrogenase (LDH) release assay is a sensitive and effective method for assessing cell viability in short-term cultures, outperforming the trypanblue exclusion test in detecting various cellular responses.
Area of Science:
- Cellular biology
- Biochemistry
- Toxicology
Background:
- Cell viability assays are crucial for evaluating the effects of substances on cells.
- The trypanblue exclusion test and lactate dehydrogenase (LDH) release assay are commonly used methods.
- Comparing their sensitivity and correlation is important for optimizing experimental design.
Purpose of the Study:
- To compare the correlation and sensitivity of the trypanblue exclusion test and LDH release assay.
- To investigate different cellular reaction types induced by tissue extracts and digitonin.
- To evaluate the suitability of LDH release assay for short-term cell cultures.
Main Methods:
- Short-term cultures (4 hours at 37°C) of human blood leucocytes.
- Assay of cell viability using trypanblue exclusion test and LDH release assay.
- Exposure to varying doses of tissue extracts or digitonin.
Main Results:
- Identified three reaction types: synchronic (analogous or negative dose-dependent reactions), asynchronic (trypanblue negative, increased LDH release indicating permeability changes), and stabilization (suppressed LDH release).
- The trypanblue exclusion test failed to detect cell stabilization effects.
- LDH release assay demonstrated sensitivity and objectivity, especially with automated measurement.
Conclusions:
- The LDH release assay is a sensitive, objective, and effective method for short-term cell cultures.
- It can detect various cellular responses, including stabilization, which trypanblue exclusion cannot.
- LDH release assay is recommended for testing pharmaceuticals, antigens, allergens, and biomaterials in vitro.