Catecholamines and serotonin in rat frontal cortex after PCPA and 6-OHDA: absolute amounts and ratios

The catecholamines dopamine, epinephrine and norepinephrine, as well as the indoleamine serotonin were measured using radioenzymatic assays, in the frontal cortex of normal rats and of rats in which serotonin synthesis had been inhibited with p-chlorophenylalanine. Besides serotonin, norepinephrine levels were significantly reduced, thus changing the monoamine ratios. To further evaluate these changes, two models of catecholaminergic deafferentation were analyzed, i.e., the massive catecholamine depletion induced by intraventricular 6-OHDA and the more selective norepinephrine deafferentation, obtained by microinjecting 6-OHDA in the dorsal noradrenergic bundle. The results are interpreted in relation to the functional interactions between the catecholamines and between the catecholamines and serotonin.