Related Experiment Videos
A human lymphocyte chemotactic factor produced by the mixed lymphocyte reaction
The Journal of Laboratory and Clinical Medicine
|October 1, 1982
Summary
Researchers discovered a human T lymphocyte chemotactic factor produced during mixed lymphocyte reactions (MLR). This factor attracts T cells to antigenic sites, potentially explaining lymphocyte migration in immune responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- Lymphocyte migration to antigenic sites is crucial for immune responses like delayed hypersensitivity and graft rejection.
- The precise mechanisms attracting lymphocytes to these sites remain incompletely understood.
Purpose of the Study:
- To investigate the production and characteristics of lymphocyte chemotactic factors during human mixed lymphocyte reactions (MLR).
- To determine if MLR supernatants attract T lymphocytes and influence their locomotion.
Main Methods:
- Human mononuclear cells were cultured in a two-way MLR for 7 days.
- Supernatants were collected and assayed for lymphocyte chemotactic activity.
- Heat lability and effects on T cell locomotion (chemotaxis and chemokinesis) were evaluated.
Main Results:
- A heat-labile lymphocyte chemotactic factor was detected in MLR supernatants, with peak activity at 24-48 hours.
- This factor preferentially attracted T lymphocytes.
- MLR supernatants induced both chemotaxis and chemokinesis in T cells.
Conclusions:
- Mixed lymphocyte reactions generate a human T lymphocyte chemotactic factor.
- This factor may play a significant role in recruiting T lymphocytes to sites of antigenic challenge.
- Understanding this mechanism could offer insights into immune cell trafficking and inflammatory processes.