Enhancement by BCG of macrophage-like suppressor cells that inhibit the expression of cytotoxic T-cell effector function
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The expression of alloimmune splenic T-cell mediated cytotoxicity, as determined by the 51Cr release assay, in animals undergoing allograft rejection, was observed to be inhibited by a cell present in the plastic-adherent fractions of the spleens. The activity of the suppressor cell, induced by the presence of the allograft, was enhanced if the animals were treated with BCG: The latter also potentiated the rejection response. The suppressor cell acted at the cytolytic phase of the effector function so that there was a measurable increase in the response if the suppressor cells were removed from the immune spleens prior to the assay. The cell responsible for the suppressor activity was macrophage-like in its characteristics, being of light density, adherent to glass surfaces, Thy-1-negative and sensitive to the toxic effects of crystalline silica particles.
The expression of alloimmune splenic T-cell mediated cytotoxicity, as determined by the 51Cr release assay, in animals undergoing allograft rejection, was observed to be inhibited by a cell present in the plastic-adherent fractions of the spleens. The activity of the suppressor cell, induced by the presence of the allograft, was enhanced if the animals were treated with BCG: The latter also potentiated the rejection response. The suppressor cell acted at the cytolytic phase of the effector function so that there was a measurable increase in the response if the suppressor cells were removed from the immune spleens prior to the assay. The cell responsible for the suppressor activity was macrophage-like in its characteristics, being of light density, adherent to glass surfaces, Thy-1-negative and sensitive to the toxic effects of crystalline silica particles.