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Cefoperazone: regression analysis, disk content, and disk susceptibility testing considerations.

D N Wright, D F Welch, B A Saxon

    Antimicrobial Agents and Chemotherapy
    |September 1, 1982
    PubMed
    Summary
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    Disk diffusion testing for cefoperazone susceptibility can be optimized using specific disk concentrations and zone sizes. A 50 or 75 microgram disk is recommended for accurate bacterial strain differentiation based on minimal inhibitory concentration (MIC) values.

    Area of Science:

    • Microbiology
    • Antimicrobial Susceptibility Testing

    Background:

    • Accurate antimicrobial susceptibility testing is crucial for effective treatment.
    • Disk diffusion and agar dilution are common methods for determining bacterial susceptibility.
    • Cefoperazone is a valuable antibiotic, necessitating reliable susceptibility testing methods.

    Purpose of the Study:

    • To compare cefoperazone agar dilution minimal inhibitory concentration (MIC) results with disk diffusion zone of inhibition sizes.
    • To evaluate the efficacy of different cefoperazone disk concentrations (30, 50, 75, 100 micrograms) for susceptibility testing.
    • To establish optimal disk diffusion parameters for predicting cefoperazone susceptibility and resistance.

    Main Methods:

    • Agar dilution method was used to determine minimal inhibitory concentrations (MICs).

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  • Disk diffusion tests were performed using cefoperazone disks of varying concentrations.
  • Regression line comparisons and error rate-bounded analysis were conducted.
  • Zone sizes for susceptibility (S) and resistance (R) were correlated with MIC values.

    • Main Results:

    • For an MIC susceptibility breakpoint of 32 µg/ml, a 50 µg disk (S ≥ 15 mm, R ≤ 12 mm) or a 75 µg disk (S ≥ 17 mm, R ≤ 14 mm) showed potential utility.
    • For an MIC susceptibility breakpoint of 64 µg/ml, a 75 µg disk (S ≥ 17 mm, R ≤ 12 mm) was indicated.
    • Disks with ≤30 µg or ≥100 µg of cefoperazone did not effectively differentiate susceptible from resistant strains at either MIC breakpoint.

    Conclusions:

    • The choice of cefoperazone disk concentration and corresponding zone size breakpoints is critical for accurate susceptibility testing.
    • 50 µg and 75 µg cefoperazone disks demonstrate potential for reliable bacterial strain differentiation.
    • Further validation is needed to confirm optimal disk diffusion parameters for clinical use.