[Reconstitution of the function of the coupling complex of chloroplasts on phospholipid vesicles]

The function of H+-ATPase (CF1-CF0) complex from spinach chloroplasts was reconstituted on azolectin liposomes. Using these proteoliposomes, the 32P-ATP exchange reaction sensitive to dicyclohexylcarbodiimide and uncouplers was established. Combined incorporation of H+-ATPase and bacteriorhodopsin as a delta mu H+ inducer results in photophosphorylation. The optimal conditions for production of liposomes with a low permeability of membranes for ions were elaborated with a view towards improving the existing reconstitution methods.