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Related Experiment Videos

[Colonic nerve network demonstrated by quinacrine].

D Gaillard, P Birembaut, D Ploton

    Bulletin De L'Association Des Anatomistes
    |March 1, 1982
    PubMed
    Summary
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    Quinacrine staining reveals a distinct nerve network in the gut. The Piebald mouse model mimics Hirschsprung

    Area of Science:

    • Neuroscience
    • Gastroenterology
    • Histology

    Context:

    • The myenteric plexus, a component of the enteric nervous system, regulates gastrointestinal motility.
    • Congenital megacolon and Hirschsprung's disease involve abnormalities in the enteric nervous system.
    • Quinacrine is a fluorescent dye used to visualize specific neuronal populations.

    Purpose:

    • To characterize the quinacrine-positive nerve network in the myenteric plexus of the Piebald mouse.
    • To compare the quinacrine-positive network in the Piebald mouse model with human Hirschsprung's disease.
    • To investigate the relationship between the quinacrine-positive network and cholinergic/adrenergic fibers in both models.

    Summary:

    • Quinacrine staining identified a unique nerve network within the myenteric plexus, distinct from cholinergic and adrenergic systems.

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  • In Piebald mice (congenital megacolon model), this network was reduced in the distal segment and developed proximally.
  • Hirschsprung's disease showed similar quinacrine network alterations but with increased extrinsic cholinergic and adrenergic fibers, suggesting a pseudo-Hirschsprung's phenotype in the mouse model.

    • Impact:

    • The Piebald mouse serves as a valuable model for studying pseudo-Hirschsprung's disease, a condition not previously reported in human pathology.
    • This research provides insights into the complex organization of the enteric nervous system and its role in congenital gut motility disorders.
    • Understanding these neuronal networks can inform future therapeutic strategies for Hirschsprung's disease and related conditions.