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Inhibition of autologous mixed lymphocyte reaction by aggregated IgG molecules
Human autologous and allogeneic mixed lymphocyte reactions (MLR) were studied using various T cell subsets as responding cells. T cells expressing Fc gamma receptor for IgG (TG) were weak responder while T cells with Fc mu receptor and T cells lacking Fc receptor responded vigorously in both types of MLR. Percoll-separated T cells of low density (TL) enriched for TG cells were, however, excellent responders in autologous MLR, whereas T cells of high density (TH) were the major responders in allogeneic MLR. Continuous presence of or short-term pulse with aggregated IgG significantly reduced the autologous MLR of unfractionated T and TL cells while largely sparing proliferation in allogenic MLR. These findings are discussed with respect to diminished autologous MLR reactivity in certain autoimmune diseases.
Human autologous and allogeneic mixed lymphocyte reactions (MLR) were studied using various T cell subsets as responding cells. T cells expressing Fc gamma receptor for IgG (TG) were weak responder while T cells with Fc mu receptor and T cells lacking Fc receptor responded vigorously in both types of MLR. Percoll-separated T cells of low density (TL) enriched for TG cells were, however, excellent responders in autologous MLR, whereas T cells of high density (TH) were the major responders in allogeneic MLR. Continuous presence of or short-term pulse with aggregated IgG significantly reduced the autologous MLR of unfractionated T and TL cells while largely sparing proliferation in allogenic MLR. These findings are discussed with respect to diminished autologous MLR reactivity in certain autoimmune diseases.