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Related Experiment Videos

A second function of the S gene of bacteriophage lambda.

D B Wilson, A Okabe

    Journal of Bacteriology
    |December 1, 1982
    PubMed
    Summary

    Bacteriophage lambda infection of Escherichia coli inhibits active transport and increases inner membrane permeability. Recovery of transport activity requires a specific phage protein, highlighting its role in cellular processes.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Cellular Biology

    Background:

    • Escherichia coli (E. coli) active transport systems are crucial for nutrient uptake.
    • Bacteriophages are viruses that infect bacteria, potentially altering host cell functions.

    Purpose of the Study:

    • To investigate the immediate and transient effects of bacteriophage lambda infection on E. coli active transport systems.
    • To identify the mechanisms and phage components involved in transport inhibition and recovery.

    Main Methods:

    • Monitoring uptake of various solutes (sucrose, glycine, alpha-methyl glucoside) in E. coli post-infection.
    • Measuring intracellular ATP levels and membrane permeability.
    • Analyzing the role of phage DNA injection and specific phage proteins using mutant phages.

    Main Results:

    • Lambda phage infection rapidly inhibited most active transport systems and increased membrane permeability to small molecules but not nucleotides.
    • ATP levels dropped significantly, but DNA injection, not mere phage adsorption, triggered inhibition.
    • Transport inhibition was transient, with recovery dependent on a 5,500 MW lambda protein; mutant phages lacking this protein showed impaired recovery.

    Conclusions:

    • Bacteriophage lambda infection profoundly impacts E. coli membrane transport functions.
    • A specific lambda phage structural protein is essential for the recovery of active transport post-infection.
    • These findings elucidate phage-host interactions at the membrane transport level.

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