Rapid method for the simultaneous analysis of hydrocortisone and clioquinol in topical preparations by...

Reversed-phase high performance liquid chromatographic methods for the analysis of ointments containing hydrocortisone and clioquinol have been investigated. A successful method using a C18 column and methanol--0.05 M phosphoric acid (80:20) as eluting solvent has been developed which allows both compounds to be determined simultaneously. The high-performance liquid chromatographic procedure is rapid and sensitive whereas the assay described in the 1980 BP involves a different method for the analysis of each component of the ointment. The method has also been further applied to the analysis of ointments containing hydrocortisone combined with other halogenated hydroxyquinolines.