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Lethal action of bacteriophage lambda S gene.

J M Garrett, R Young

    Journal of Virology
    |December 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Bacteriophage lambda lysis genes S, R, and Rz were studied. Gene S product is stable and triggers cell lysis, while Rz is essential for Mg2+-dependent lysis.

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    Area of Science:

    • * Molecular biology
    • * Virology
    • * Genetics

    Background:

    • * Bacteriophage lambda utilizes specific lysis genes for host cell destruction.
    • * Previous work suggested a role for the Rz gene in the lysis process.

    Purpose of the Study:

    • * To investigate the functions of bacteriophage lambda lysis genes S, R, and Rz.
    • * To confirm the involvement of Rz in lysis and elucidate the mechanism of S action.

    Main Methods:

    • * Cloning and expression of wild-type and inactive alleles of lysis genes S, R, and Rz in a plasmid.
    • * Analysis of lysis defects in gene-deleted clones.
    • * Measurement of active glucose transport in membrane vesicles from induced cells.
    • * Assessment of cell viability following gene induction.

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    Main Results:

    • * Deletion of part of the Rz gene resulted in a magnesium-dependent lysis defect, confirming Rz's role.
    • * Induced S+ cells exhibited reduced glucose transport capacity prior to lysis.
    • * Cell viability decreased rapidly after induction, dependent on S expression but independent of R and Rz.
    • * The S gene product is stable and can induce lysis even after its synthesis stops.

    Conclusions:

    • * The S gene product is a key, stable factor initiating cell lysis.
    • * Rz plays a crucial role in magnesium-dependent lysis.
    • * The S gene product's action precedes and is independent of R and Rz functions in the lysis cascade.