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Related Experiment Videos

Induction of c-mutations in extracellular phage lambda by gamma-rays.

S E Bresler, V L Kalinin, I N Suslova

    Molecular & General Genetics : MGG
    |January 1, 1982
    PubMed
    Summary

    Gamma-rays induce premutational lesions in phage DNA. The inducible SOS-repair system converts these lesions into mutations, especially in broth conditions, enhancing mutagenic action.

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    Area of Science:

    • Molecular Biology
    • Radiation Biology
    • Genetics

    Background:

    • Extracellular phages are susceptible to DNA damage from ionizing radiation.
    • The SOS-repair system in host cells plays a role in DNA repair and mutagenesis.

    Purpose of the Study:

    • To investigate the mutagenic effects of cobalt-60 gamma rays on extracellular phages.
    • To determine the influence of radiation conditions and host cell repair mechanisms on phage mutagenesis.

    Main Methods:

    • Irradiation of phages lambda red1 and lambda red113 in nutrient broth, with or without cysteamine, and in dried samples.
    • Assessing mutation yields across various host cell repair genotypes (uvrA6, polA1, recA13, lexA102, uvrE502, uvrD3, xthA9).
    • Induction of the SOS-repair system in host cells using UV irradiation prior to phage infection.

    Main Results:

    • Mutation yield was largely independent of host repair genotype, phage Red function, and irradiation conditions.
    • SOS-repair induction significantly enhanced mutation yield for phages irradiated in broth, but not in dried samples.
    • Gamma-ray-induced premutational lesions are fixed by replication or by the error-prone SOS-repair system.

    Conclusions:

    • Direct gamma-ray action generates premutational lesions in phage DNA.
    • The SOS-repair system is a major pathway for converting these lesions into mutations, particularly under conditions allowing indirect radiation effects.

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