[Acute ischemic hypoxia of the liver]

The author studied some aspects related to the acute ischaemic hypoxia of the liver using an original methodology based on the selective in situ perfusion of the liver of dogs. Hypoxia was obtained by clamping the liver pediculum for a duration of 30 minutes. In a first group of experiments the dynamics of the carbohydrate metabolism during hypoxia was followed. On this occasion was established the use of some washing solutions containing large amounts of substances involved in the protection of the hepatocytes from the viewpoint of their glycogenic stores in the course of the sampling of the liver grafts. This has important effects in maintaining the biological qualities of the organ. In a second group of experiments the involvment of the Kupffer-cell system was investigated, as a result of ischaemic hypoxia. A colloidopexia test was used, which employed 198Au. In view of obtaining an increased tolerance to hypoxia selective perfusions were carried out, with a solution containing cortison hemisuccinate and Essentiale 303. The results confirmed the lysosome-protecting effects of this solution when perfusion preceded ischaemia by 90-120 minutes. It also confirmed the test value of the colloido-pexia method. For the first time it was confirmed that there is a significant increase in the 198Au fixation in the spleen in the animals the liver of which was exposed to ischaemia.