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Monoclonal anti-(T,G)-A--L antibodies: characterization of fine specificity and idiotype expression
The technique of polyethylene glycol mediated cell fusion was used to establish 22 monoclonal cell lines secreting anti-(T,G)-A--L antibody. Cell lines were derived from C3H.SW and B10 mice and produced antibody with kappa light chains and predominantly gamma 1, heavy chains. Fine-specificity analysis demonstrated that 15 cell lines made antibodies that also recognize a determinant present on GAT, GT (9:1) and GT (1:1), whereas little, if any, serum antibody demonstrates this cross-reaction. Fourteen antibodies, derived from both B10 and C3H.SW mice, bear idiotypic determinants defined by Lewis anti-[B10 anti-(T,G)-A--L], but only two, both from C3H.SW mice, react with Lewis anti-[C3H.SW anti-(T,G)-A--L]. Adsorption studies indicate that no hybridoma tested bore the complete set of idiotypic determinants defined by either serum.
The technique of polyethylene glycol mediated cell fusion was used to establish 22 monoclonal cell lines secreting anti-(T,G)-A--L antibody. Cell lines were derived from C3H.SW and B10 mice and produced antibody with kappa light chains and predominantly gamma 1, heavy chains. Fine-specificity analysis demonstrated that 15 cell lines made antibodies that also recognize a determinant present on GAT, GT (9:1) and GT (1:1), whereas little, if any, serum antibody demonstrates this cross-reaction. Fourteen antibodies, derived from both B10 and C3H.SW mice, bear idiotypic determinants defined by Lewis anti-[B10 anti-(T,G)-A--L], but only two, both from C3H.SW mice, react with Lewis anti-[C3H.SW anti-(T,G)-A--L]. Adsorption studies indicate that no hybridoma tested bore the complete set of idiotypic determinants defined by either serum.